作者：赵轶峰1，李明霞2，张超3，胡晓敏1，王雄1，赵铁军1

单位：1.河北北方学院附属第一医院 胃肠肿瘤外科，河北  张家口 075000; 2.河北北方学院附属第一医院 内分泌与代谢病科，河北 张家口 075000; 3.河北北方学院附属第一医院 介入科，河北 张家口075000

Authors: Zhao Yifeng1，Li Mingxia2，Zhang Chao3，Hu Xiaomin1，Wang Xiong1，Zhao Tiejun1

Unit: 1.Department of Gastrointestinal Tumor Surgery，the First Hospital Affiliated to Hebei North University，Zhangjiakou 075000，Hebei，China; 2.Department of Internal Medicine and Metabolic Diseases，the First Hospital Affiliated to Hebei North University，Zhangjiakou 075000，Hebei，China; 3.Department of Interventional，the First Hospital Affiliated to Hebei North University，Zhangjiakou 075000，Hebei，China

摘要：

目的 探讨微小RNA-433-3p(miR-433-3p)靶向同源异形盒基因A1 (HOXA1)对胃癌MGC-803细胞增殖、侵袭的影响。方法 体外培养人胃癌MGC-803细胞，分为对照组（正常培养，不进行任何处理）、si-NC组（转染miR-433-3p siRNA阴性对照）、si-miR-433-3p组（转染miR-433-3p siRNA）、mimic-NC组（转染miR-433-3p mimic阴性对照）和miR-433-3p mimic组（转染miR-433-3p mimic）。荧光定量聚合酶链反应（PCR）法检测MGC-803细胞中miR-433-3p及HOXA1 mRNA表达；细胞计数法、Transwell法分别检测MGC-803细胞增殖、侵袭；双荧光素酶报告基因试验检测miR-433-3p与HOXA1的靶向关系。结果 与对照组和si-NC组相比，si-miR-433-3p组MGC-803细胞中miR-433-3p表达降低（P<0.05）,HOXA1 mRNA表达、细胞增殖率、侵袭细胞数升高（P<0.05）。与对照组和mimic-NC组相比，miR-433-3p mimic组MGC-803细胞中miR-433-3p表达升高（P<0.05）,HOXA1 mRNA表达、细胞增殖率、侵袭细胞数降低（P<0.05）。TargetScan网站预测及双荧光素酶报告基因试验结果显示，miR-433-3p与HOXA1在MGC-803细胞中存在靶向关系。结论 miR-433-3p能靶向调控HOXA1抑制胃癌MGC-803细胞的增殖、侵袭过程。 

关键词：微小RNA-433-3p；同源异形盒基因A1；胃癌MGC-803 细胞；增殖；侵袭

Abstract：

Objective To investigate the effects of microRNA-433-3p （miR-433-3p ） targeting homeobox gene A1 （HOXA1） on the proliferation and invasion of gastric cancer MGC-803 cells. Method Human gastric cancer cells MGC-803 were cultured in vitro and divided into control group ，si-NC group，simiR-433 -3p group，mimic -NC group，and miR-433 -3p mimic group. Real -time fluorescent quantitative PCR method was used to detect the expression of miR-433-3p and HOXA1 mRNA in MGC-803 cells. Cell counting，Transwell method were used to detect the proliferation，invasion of MGC -803 cells. The dual luciferase reporter gene experiment was used to detect the targeting relationship between miR-433 -3p and HOXA1. Result Compared with the control group and si-NC group，the expression level of miR-433-3pin MGC-803 cells in the si-miR-433-3p group reduced （P<0.05），the HOXA1 mRNA expression level，cell proliferation rate ，number of invasive cells increased （P<0.05）. Compared with the control group and mimic-NC group，the expression level of miR-433-3p in MGC-803 cells in the miR-433-3p mimic group increased significantly（P<0.05），the HOXA1 mRNA expression level，cell proliferation rate，number of invasive cells reduced （P<0.05）. TargetScan website prediction and dual luciferase reporter gene experiment results showed that miR-433-3p and HOXA1 had a targeting relationship in MGC-803 cells. Conclusion miR-433-3p can target HOXA1 to inhibit the proliferation and invasion of gastric cancer MGC-803 cells.

Key Words: MicroRNA-433-3p;  Homeobox gene A1;  Gastric MGC-803 cancer;  Proliferation;  Invasion