作者： 张伟康1，胡志耀1，钟剑锋1，姜德培1，文学1，王新友2，陈泓磊1

单位：1.中山大学附属第八医院（深圳福田） 消化内镜中心，广东 深圳 518033；2.中山大学附属第六医院 胃外科，广东 广州 510655

Authors:  Zhang Weikang1，Hu Zhiyao1, Zhong Jianfeng1，Jiang Depei1，Wen Xue1，Wang Xinyou2，Chen Honglei1

Unit:  1.Gastrointestinal Endoscopy Center, the Eighth Affiliated Hospital of Sun Yat-sen University, Shenzhen 518033, Guangdong, China；2.Gastrointestinal Surgery, the Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou 510655, Guangdong, China

摘要：

目的 探讨BLM基因与胃癌组织中免疫细胞浸润水平及患者预后的关系。方法 通过TIMER数据库分析BLM基因在不同恶性肿瘤中的表达水平差异，并应用GEPIA数据库验证BLM基因在胃癌组织中的表达水平。对胃癌组织芯片（STC1602）进行免疫组织化学分析，并对比胃癌组织和正常胃黏膜组织BLM免疫组织化学结果，进一步验证BLM在胃癌组织中的表达差异。通过Kaplan-Meier Plotter数据库对BLM基因与胃癌患者的生存预后关系进行评估。利用TIMER数据库分析了BLM基因在胃癌组织中的表达与免疫细胞浸润水平的相关性。从基因表达综合数据库中获取了GSE84433和GSE62254胃癌数据集，进行了单样本基因集富集分析，以验证BLM基因的高表达是否会影响胃癌组织中免疫细胞的浸润水平。结果 BLM基因在胃癌、结直肠癌、食管癌等恶性肿瘤组织中均高表达。BLM基因高表达水平的胃癌患者的总生存和首次进展后生存均较差。胃癌组织中BLM基因的mRNA表达水平与CD8+ T细胞、CD4+ T细胞、巨噬细胞、中性粒细胞及树突状细胞的浸润水平呈负相关。结论 BLM基因在胃癌组织中高表达，BLM基因的表达与胃癌组织中的免疫细胞浸润水平呈负相关，BLM基因可作为评估胃癌患者预后的标志物。

关键词： 胃癌；BLM基因；临床预后；免疫浸润

Abstract：

Objective   To investigate the relationship between BLM gene and immune cell infiltration level in gastric cancer tissues and prognosis of gastric cancer patients. Method  The expression levels of BLM gene in different malignant tumors were analyzed by the TIMER database, and the expression level of BLM gene in gastric cancer tissues was verified by GEPIA database. Besides, immunohistochemistry analysis was conducted on gastric cancer tissue microarray (STC1602), and the immunohistochemistry results of BLM between gastric cancer tissues and normal gastric mucosal tissues were compared to further verify the different expression of BLM in gastric cancer tissues. The prognostic relationship between the BLM gene and the survival of gastric cancer patients was assessed using the Kaplan-Meier Plotter database. Besides, the TIMER database was utilized to analyze the correlation between BLM gene expression in gastric cancer obtained from the gene expression omnibus database and single-sample gene set enrichment analysis was conducted to validate whether the high expression of the BLM gene affects the level of immune cell infiltration in gastric cancer tissues. Result  BLM gene was highly expressed in gastric cancer, colorectal cancer, esophageal cancer and other malignant tumor tissues. Gastric cancer patients with high expression level of BLM gene had poorer overall survival and first progression survival. The mRNA expression level of BLM gene in gastric cancer tissues was negatively correlated with the infiltration levels of CD8+ T cells, CD4+ T cells, macrophages, neutrophils and dendritic cells. Conclusion  BLM gene is highly expressed in gastric cancer tissues, and the expression of BLM gene is negatively correlated with immune cell infiltration levels in gastric cancer tissues, and BLM gene can be used as a marker to evaluate the prognosis of gastric cancer patients.

Key Words:  Gastric cancer; BLM gene; Clinical prognosis; Immune infiltration

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