The role of osteoglobin in the maintenance of gastric cancer cell stemness and sensitivity to chemotherapy
作者：邹岩1，姜桂春1，李辉2，谢艳敏1
单位：1.辽宁省肿瘤医院 内镜中心, 辽宁 沈阳 110042;
2.辽宁省肿瘤医院 护理部, 辽宁 沈阳 110042
Authors: Zou Yan1，Jiang Guichun1，Li Hui2，Xie Yanmin1
Unit: 1. Endoscopy Center，Liaoning
Province Cancer Hospital & Institute，Shenyang
110042，Liaoning，China; 2.
Nursing Department，Liaoning Province Cancer Hospital
& Institute，Shenyang 110042，Liaoning，China
摘要：
目的 探究骨甘蛋白（OGN）对胃癌（GC）细胞干性与化疗耐药性的影响。方法 实时荧光聚合酶链反应（RT-PCR）、蛋白质印迹法、免疫组织化学（IHC）联合癌症基因组图谱（The Cancer Genome Atlas,TCGA）数据库分析OGN在胃癌细胞和组织中的表达。CCK-8和克隆形成评估细胞增殖能力变化，通过流式细胞术对细胞凋亡进行测定，CCK-8检测OGN在GC细胞化疗抵抗中的作用。蛋白质印迹法检测细胞干性相关分子标志物表达变化。结果 OGN在GC细胞表达低于其在胃黏膜细胞中表达（P<0.05）；过表达OGN的GC细胞侵袭能力减弱（P<0.05），凋亡增加（P<0.05）。在铂类和氟尿嘧啶处理后，细胞存活比例下降，化疗抵抗减轻（P<0.05）,OGN诱导了化疗耐药。过表达OGN抑制了干性标志物的表达。结论 OGN在GC组织中低表达，过表达的OGN能够抑制细胞干性，影响化疗耐药。
关键词：胃癌； 骨甘蛋白； 增殖； 凋亡； 干性； 化疗耐药
Abstract：
Objective To investigate the
effect of osteoglycin (OGN) on the stemness and chemotherapy resistance of
gastric cancer (GC) cells. Method Real-time polymerase chain reaction
(RT-PCR)，western-blot，immunohistochemistry
(IHC) combined with The Cancer Genome Atlas (TCGA) database to analyze OGN in
GC cells and tissues expression. CCK-8 and clone formation assay were used to
assess changes in cell proliferation ability，and
apoptosis was measured by flow cytometry. CCK-8 was used to detect the role of OGN
in chemoresistance of GC cells. Western-blot detection of cell stemness
-related molecular markers expression changes. Result The expression of
OGN in GC cells was lower than that in gastric mucosal cells (P<0.05).
Overexpressed OGN decreased the invasive ability of GC cells (P<0.05) and
increased apoptosis (P <0.05). After platinum and fluorouracil treatment，the survival rate of cells decreased and chemotherapy resistance was
alleviated (P<0.05)，and OGN induced chemotherapy
resistance . Overexpression of OGN suppressed the expression of stemness
markers. Conclusion OGN is weakexpressed in GC tissues，and overexpressed OGN can inhibit cell stemness and affect
chemotherapy resistance.
Key Words: Gastric
cancer; Osteoglycin; Proliferation; Apoptosis; Stemness; Chemoresistance
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