作者：邓镇生1，张展飞2，许静红3，杨佳丽4，刘振国4

单位：1.三亚中心医院（海南省第三人民医院） 心胸外科， 海南 三亚 572000；2.中山市人民医院 心胸外科， 广东 中山 528403；3.三亚中心医院（海南省第三人民医院） 麻醉科， 海南 三亚 572000；4.中山大学附属第一医院 胸外科， 广东 广州 510080

Authors: Deng Zhensheng1, Zhang Zhanfei2, Xu Jinghong3, Yang Jiali4, Liu Zhenguo4

Unit: 1. Cardiothoracic Surgery, Sanya Central Hospital, (Hainan Third People's Hospital) Sanya 572000, Hainan, China;  2.Cardiothoracic Surgery, Zhongshan People's Hospital, Zhongshan 528403, Guangdong, China;  3.Department of Anesthesiology, Sanya Central Hospital, (Hainan Third People's Hospital) Sanya 572000, Hainan, China;  4.Thoracic Surgery, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong, China

摘要：

目的 检测食管鳞状细胞癌（简称鳞癌）组织中吞噬和运动蛋白1 (ELMO1)的表达情况，并探讨ELMO1与食管鳞癌侵袭转移、临床分期及预后的关系。方法 选取2013年1月至2015年1月广州市中山大学附属第一医院食管癌手术标本86例，应用免疫组织化学和蛋白质印迹法检测食管鳞癌组织中ELMO1的表达情况，并结合肿瘤基因组图谱数据库分析ELMO1的表达水平与临床特征、分期及预后的相关性。结果 ELMO1蛋白在食管鳞癌组织中呈现不同程度的高表达。ELMO1的表达水平与pT、pN和TNM分期正相关（P<0.05）,ELMO1表达越高，TNM分期越高。Kaplan-Meier生存曲线分析显示,与ELM01低表达组比较，ELMO1高表达组患者5年生存更差（P<0.05）。结论 食管鳞癌中ELMO1表达水平与pT、pN、TNM分期及预后生存相关，ELMO1可作为食管鳞癌预后预测的潜在标志物。

关键词：食管鳞癌； 吞噬和运动蛋白1； TNM分期； 预后

Abstract：

Objective To detect the expression of engulfment and cell mobility 1 （ELMO1） in esophageal squamous cell carcinoma (ESCC), and to investigate the relationship between ELMO1 and lymph node metastasis, clinical stage and survival prognosis of ESCC. Method 86 cases of esophageal cancer patients from the First Affiliated Hospital of Sun Yat-sen University were enrolled from January 2013 to January 2015. ELMO1 protein expression in ESCC specimens was detected by immunohistochemical (IHC) and Western blot. Based on TCGA database and our data, the relationship between the expression of ELMO1 and the clinical characteristics, staging and prognosis of esophageal cancer were analyzed. Result ELMO1 protein was over expressed in esophageal squamous cell carcinoma to varying degrees. The expression level of ELMO1 was significantly correlated with pT, pN and TNM stage(P<0.05). Kaplan-Meier survival curve analysis showed that the 5 -year survival of patients with high ELMO1 expression in esophageal cancer tissue was lower than patients with negative or low ELMO1 expression (P<0.05). Conclusion The expression level of ELMO1 in ESCC is significantly correlated with pT, pN, TNM stage and prognosis of survival. ELMO1 can be used as a potential prognostic biomarker for patients with ESCC.

Key Words:  Esophageal squamous cell carcinoma; Engulfment and cell mobility 1; TNM staging; Survival prognosis