作者：陈俊廷, 柳逸楠, 黄志鑫, 陈斯乐, 蔡世荣

单位：中山大学附属第一医院 胃肠外科中心，广东 广州 510080

Authors:  Chen Junting, Liu Yinan, Huang Zhixin, Chen Sile, Cai Shirong

Unit:  Department of Gastrointestinal Surgery, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangzhou, China

摘要：

目的 探讨胃癌组织中谷胱甘肽特异性γ-谷氨酰转移酶1(CHAC1)表达情况及其临床意义。方法 采用免疫组织化学法检测CHAC1在58例接受根治性手术切除的胃癌患者的胃癌组织及相应癌旁组织中的表达。应用组织芯片及免疫组织化学法检测在含有预后患者的样本中CHAC1的差异表达。结果 CHAC1在胃癌组织中的阳性率明显低于癌旁组织(P<0.05);CHAC1在经过免疫组织化学半定量评分后,通过预后分析发现:胃癌中高表达CHAC1基因的患者5年生存期较长(P<0.05)。多因素Cox回归分析得出CHAC1是胃癌的独立预后因素(P=0.001; 95%CI=0.249～0.622; HR=0.393)。结论 CHAC1在胃癌组织中高表达,且与较差的生物学行为相关。 

关键词：谷胱甘肽特异性γ-谷氨酰转移酶1；胃癌

Abstract：

Objective To investigate the expression of glutathione-specific γ-glutamyltransferase 1 （CHAC1） in gastric cancer tissues and its clinical significance. Methods The expression of CHAC1 in gastric cancer tissues and corresponding paraneoplastic tissues of 40 gastric cancer patients who underwent radical surgical resection was detected by immunohistochemistry. Tissue microarray and immunohistochemistry were applied to detect the differential expression of CHAC1 in samples containing patients with prognosis. Results The positive rate of CHAC1 in gastric cancer tissues was significantly lower than that in paraneo-plastic tissues （P<0.05）; CHAC1 was obtained by post-hoc analysis after IRS immunohistological scoring. Five-year survival was longer in patients with high expression of CHAC1 gene in gastric cancer （P<0.05）. In addition, CHAC1 was an independent prognostic factor for gastric cancer by Cox multivariate regression analysis （P=0.001; 95%CI=0.249-0.622; HR=0.393）. Conclusion CHAC1 is highly expressed in gastric cancer tissues and is associated with poorer biological behavior.

Key Words:  Glutathione-specific γ-glutamyltransferase 1; Gastric cancer