作者：吴晓滨, 罗辉兴, 翁诺卿

单位：中山大学附属第八医院 胃肠外科, 广东 深圳 518033

Authors:  Wu Xiaobin, Luo Xinghui, Weng Ruoqing

Unit:  Department of Gastrointestinal Surgery, the Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, Guangdong, 518033

摘要：

目的 探讨接头相关复合体蛋白3亚基2(adaptor related protein complex 3 subunit mu2, AP3M2)在胃癌中的表达情况及其临床意义。方法 通过TCGA数据库、实时荧光定量PCR及免疫印迹实验检测A P3M2基因的mRNA及蛋白在胃癌组织中的表达情况。通过检测KM Plotter网站探索AP3M2的表达与胃癌患者预后的关系。通过平板克隆形成实验检测AP3M2对胃癌细胞的作用。结果 TCGA数据库数据及本研究收集的胃癌患者组织显示AP3M2相比于癌旁组织,其在胃癌组织中的mRNA表达水平升高,同时AP3M2的蛋白在胃癌组织中的表达也有上调的现象。同时本研究通过KM Plotter网站分析得到AP3M2的mRNA表达水平与胃癌患者的预后有关,高表达AP3M2的胃癌患者其预后较低表达AP3M2的胃癌患者差。通过在AGS细胞(人胃腺癌细胞)中过表达AP3M2并检测其平板克隆形成能力的变化,发现过表达AP3M2可以增强胃癌细胞的克隆形成能力。结论 AP3M2在胃癌中高表达,高表达AP3M2与胃癌患者的不良预后有关,且能增强胃癌细胞的克隆形成能力。

关键词：AP3M2; 胃癌; 生存预后; 促癌作用

Abstract：

Objective To investigate the expression of AP3M2 in gastric cancer and its clinical significance. Methods The mRNA expression of AP3M2 was detected by TCGA database and RT-qPCR and the protein expression of AP3M2 was measured by western blotting. The relationship between the expression of AP3M2 and the prognosis of gastric cancer patients was explored by KM Plotter website. The effect of AP3M2 on gastric cancer cells was detected by clone formation experiment. Results The data from the TCGA database and the tissues of gastric cancer patients collected by ourselves showed that the mRNA expression level of AP3M2 in gastric cancer tissues was increased compared with the adjacent tissues, and the protein expression of AP3M2 in gastric cancer tissues was also upregulated. Meanwhile, according to the analysis of KM Plotter website, we found that the mRNA expression level of AP3M2 was related to the prognosis of gastric cancer patients, while the prognosis of gastric cancer patients with high expression of AP3M2 was worse than that of gastric cancer patients with low expression of AP3M2. Overexpressing of AP3M2 in AGS cells can enhance the clonal formation ability of gastric cancer cells. Conclusion The high expression of AP3M2 in gastric cancer is related to the poor prognosis of gastric cancer patients, and can enhance the clonogenesis ability of gastric cancer cells.

Key Words:  Adaptor related protein complex 3 subunit mu 2; Gastric Cancer; Survival prognosis