作者：戴劲1，彭铁立1，余相地2

单位：1.广州医科大学消化病研究所，广州医科大学附属第六医院（清远市人民医院） 消化内科，广东 清远 511500；2.贵州省人民医院 麻醉科，贵州 贵阳 550001

Authors:  Dai Jin1, Peng Tieli1, Yu Xiangdi2

Unit:  1.Department of Gastroenterology, the Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan，People's Hospital, Guangdong 511500，Guangdong，China；2.Department of Anesthesiology, Guizhou Provincial People’s Hospital，Guiyang 550003，Guizhou，China

摘要：

目的 探讨过表达胃动蛋白(gastrokine-2, GKN2)对胃癌细胞生物行为学的影响。方法 体外培养SGC-7901、AGS胃癌细胞,构建GKN2过表达质粒,利用Lipofectamine 2000进行GKN2过表达质粒与胃癌细胞的转染。获得GKN2过表达的SGC-7901、AGS胃癌细胞为CKN2组,空载转染上述胃癌细胞为vector组。采用分别采用细胞黏附实验、细胞侵袭实验和四甲基偶氮唑盐(MTT)法,观察GKN2对胃癌细胞黏附作用、侵袭力和细胞活性的影响。结果 与vector组相比,GKN2组GKN2的表达量明显增加(P<0.001);与vector组相比,GKN2组胃癌细胞黏附能力降低(P<0.05),胃癌细胞侵袭力明显减慢(P<0.05),胃癌细胞活性明显降低(P<0.05)。结论 GKN2能够抑制胃癌细胞黏附能力,迁移能力和细胞活性。

关键词：胃癌细胞；胃动蛋白2；细胞黏附力；细胞侵袭力；细胞活性

Abstract：

Objective This study aims to explore the effect of over-expressing gastrokine-2 (GKN2) on biological behaviors of gastric cancer cells. Methods Gastric cell lines (SGC-7901 and AGS) were cultivated in vitro, the GKN2 overexpression vector was constructed and gastric cell lines were transfected with the method of Lipofectamine 2000. GKN2 overexpression gastric cancer cell lines were experiment group (GKN2), the control group was gastric cell lines was transfected with empty vector (vector). GKN2 expression was measured by RT-PCR; the effect of GKN2 on gastric cell lines adhesion, invasion and viability was measured by adhesion assay, Transwell assay and MTT assay. Results The success of transfection was demonstrated that the expression of GKN2 in GKN2 group increased significantly by compared to vector group (P<0.05); adhesive ability of cells in GKN2 group was decreased (P<0.05); invasion ability of cells in GKN2 group was inhibited (P<0.05) and the percentage of viable cells was decreased in GKN2 group (P<0.05). Conclusion Gastrokine-2 inhibits the adhesion, invasion ability and viability rate of gastric cancer cells.

Key Words:  Gastric cell lines; Gastrokine-2; Cell adhesion ability; Cell invasiveness; Cell viability