作者：吴建龙，颜畅，邓兴明，吕国庆

单位：北京大学深圳医院 胃肠外科，广东 深圳 518036

Authors:  Wu Jianlong, Yan Chang, Deng Xingming, Lyu Guoqing

Unit:  Department of Gastrointestinal Surgery, Peking University Shenzhen Hospital, Shenzhen518036, Guangdong, China

摘要：

目的   探讨长链非编码RNA LINC00239在结直肠癌中的表达及生物学意义。方法 GEPIA2 在线数据库(http://gepia2.cancer-pku.cn)分析LINC00239在结直肠癌中的表达量,荧光定量PCR验证LINC00239在北京大学深圳医院结直肠癌生物样本库中的表达水平。小干扰RNA敲低结直肠癌细胞内LINC00239表达,通过CCK-8细胞增殖实验和Transwell检测LINC00239对结直肠癌细胞增殖、迁移及转移能力的影响及潜在的调控机制。结果  公共数据库资料和本中心结直肠癌生物样本库均提示与配对癌旁组织相比,LINC00239在结直肠癌组织中高表达。下调LINC00239的表达可显著干扰结直肠癌细胞的增殖、迁移和侵袭能力。结论  LINC00239是结直肠癌的潜在治疗靶点。

关键词：  结直肠癌; LINC00239; 增殖; 转移

Abstract：

Objective To clarify the potential role and regulatory mechanism of LINC00239 in colorectal cancer (CRC). Methods We detected the expression levels of LINC00239 in CRC tissues in a public database, and further validated its expression in CRC samples from Peking University Shenzhen Hospital, by using quantitative real-time polymerase chain reaction (RT-qPCR). Regulation of LINC00239 on CRC cells were constructed by siRNA transfection, and then cell proliferation and metastasis capacities and related pathways were identified by cell counting Kit-8 and Transwell respectively. Results Our data showed that LINC00239 was overexpressed in CRC tissues in contrast to adjacent non-tumor tissues. In addition, downregulation of LINC00668 could significantly suppress proliferation, migration and invasion. Conclusion LINC00239 might be a potential target of CRC therapy.

Key Words:  Colorectal cancer; LINC00239; Proliferation; Metastasis