作者：邹一丰1,2，刘华山1,2，戎煜明1,3，谈应鑫1,2，陈曦1,2，翁敬容1,2，范德军1,2，林绪涛1,2

单位：1.中山大学附属第六医院 结直肠肛门外科，广东 广州 510655； 2.广东省结直肠盆底疾病研究重点实验室，广东 广州 510655； 3.中山大学附属肿瘤医院 综合科，广东 广州 510610

Authors:  ZOU Yifeng1,2，LIU Huashan1,2，RONG Yuming1,3，TAN Yingxin1,2，CHEN Xi1,2，WENG Jingrong1,2，FAN Dejun1,2，LIN Xutao1,2

Unit:  1.Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou 510655, Guangdong, China； 2.Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, the Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou 510655, Guangdong, China； 3.Department of VIP Region, Cancer Center of Sun Yat-sen University, Guangzhou 510610, Guangdong, China

摘要：

目的  探究槐耳清膏对结直肠癌细胞增殖和侵袭能力的影响。方法  应用MTS、Transwell、流式细胞术及Western blot等研究槐耳清膏对人结直肠癌细胞系的抑制作用,并建立小鼠动物模型。结果  MTS表明槐耳清膏能够抑制SW480细胞增殖;Transwell表明槐耳清膏可抑制SW480侵袭能力;流式细胞术表明槐耳清膏可诱导SW480细胞凋亡;Western blot显示槐耳清膏可上调Bax,cleavage caspase-3,抑制Bcl-2等蛋白;小鼠体内皮下成瘤实验提示亦可抑制肿瘤生长。结论  槐耳清膏可抑制结直肠癌细胞的增殖及侵袭能力,并可诱导SW480细胞凋亡,其作用机制可能与激活Bcl-2/Bax/Cleaved caspase-3凋亡相关信号通路相关。

关键词：  结直肠癌; 槐耳; 免疫调节; 微环境

Abstract：

Objective  To investigate the effects of Huaier extract on the proliferation and invasion of colorectal cancer cell SW480. Methods MTS, Transwell, Flow cytometry and Western blotting assays were used to study the effects of Huaier aqueous extract on SW480 cells in vitro. The effects of Huaier aqueous extract on SW480 cells in vivo were performed on subcutaneous transplantation tumor models of human CRC cell SW480 in BALB/c Nude Mice. Results MTS assays showed that Huaier extract inhibited cell proliferation in a concentration- and time-dependent manner. The Transwell assays demonstrated that the invasion of SW480 cells was significantly inhibited after administration of Huaier extract in SW480 cells. PI-annexin-V double staining assays using flow cytometry revealed that SW480 cell apoptosis was induced by Huaier extract. The up-regulation of Bax and cleavage caspase-3 and down-regulation of Bcl-2 were observed after treatment with Huaier extract in SW480 cells via Western blotting. In vivo, the administration of Huaier extract to mice inhibited tumor growth. Conclusions The current study revealed that Huaier extract inhibited the growth and invasion and induced cell apoptosis of CRC cell SW480, possibly via activation of Bcl-2/Bax/Cleaved caspase-3 apoptosis-associated pathway.

Key Words:  Colorectal cancer; Huaier aqueous extract; Immunoregulation; Microenvironment