作者：袁锡裕，李庆贤，黄东，龚时文

单位：南方医科大学附属东莞市人民医院 普外科，广东 东莞 523000

Authors:  YUAN Xiyu, LI Qingxian, HUANG Dong, GONG Shiwen

Unit:  Department of General Surgery, Affiliated Dongguan People’s Hospital, Southern Medical University, Dongguan 523000, Guangdong, China

摘要：

目的  检测胃癌患者血清中miR-101、miR-204的表达水平,探讨其与胃癌患者临床病理特征及幽门螺旋杆菌(Hp)感染的关系,评价血清miR-101、miR-204对胃癌的诊断价值,为胃癌的临床诊断提供参考。方法  选取东莞市人民医院普外科2016年5月～2018年2月收治的72例胃癌患者作为此次研究的观察组,另选取70例慢性浅表性胃炎患者作为此次研究的对照组。检测两组患者Hp感染情况以及血清中miR-101、miR-204的表达水平。分析血清miR-101、miR-204表达水平与Hp感染以及胃癌患者临床病理特征的关系,采用受试者工作特征曲线(ROC)分析其对胃癌的诊断价值。结果  观察组血清miR-101、miR-204表达水平均明显低于对照组,差异有统计学意义(P<0.05)。两组Hp感染为阳性的患者血清miR-101、miR-204表达水平均明显低于Hp感染为阴性的患者,差异有统计学意义(P<0.05)。观察组患者中有淋巴结转移的患者以及TNM分期为III～IV期的患者的血清miR-101、miR-204表达水平明显降低(P<0.05)。血清miR-101诊断胃癌的敏感度和特异度分别为71.35%和84.61%,血清miR-204诊断胃癌的敏感度和特异度分别为70.41%和82.83%,血清miR-101联合miR-204诊断胃癌的敏感度和特异度分别为73.76%和88.62%。结论  血清中miR-101、miR-204的表达水平在Hp感染为阳性以及病情程度较为严重的胃癌患者中明显降低,其可作为胃癌诊断的新型标志物。

关键词： 血清; miR-101; miR-204; 胃癌; 诊断; 幽门螺旋杆菌; 感染

Abstract：

Objective  To observe the expression of miR-101 and miR-204 in the serum of gastric cancer patients, to explore the relationship with the clinical features of gastric cancer patients and the infection of Helicobacter pylori (Hp), to evaluate the diagnostic value of serum miR-101 and miR-204 in gastric cancer, and provide reference for clinical diagnosis of gastric cancer. Methods 72 cases of gastric cancer who were treated in our hospital from May 2016 to February 2018 were selected as the observation group of this study, and 70 cases of chronic superficial gastritis were selected as the control group. The Hp infection and the expression levels of miR-101 and miR-204 in the two groups were detected. The serum levels of miR-101 and miR-204 in the observation group and the control group, receiver operating characteristic curve (ROC) was used to analyze the diagnostic value of gastric cancer. Results The serum levels of miR-101 and miR-204 in the observation group were significantly lower than those in the control group, the differences are statistically significant (P<0.05). In the two groups, the serum miR-101 and miR-204 levels of the patients with positive Hp infection were significantly lower than those with negative Hp infection, and the differences were statistically significant (P<0.05). The levels of miR-101 and miR-204 in patients with lymph node metastasis in the observation group and those with TNM stage III~IV stage were significantly decreased (P<0.05). The sensitivity and specificity of serum miR-101 in the diagnosis of gastric cancer were 71.35% and 84.61% respectively. The sensitivity and specificity of serum miR-204 in the diagnosis of gastric cancer were 70.41% and 82.83% respectively. The sensitivity and specificity of serum miR-101 combined with miR -204 for the diagnosis of gastric cancer were 73.76% and 88.62%, respectively. Conclusion The expression levels of miR-101 and miR-204 in serum are significantly lower in patients with positive Hp infection and more severe patients with gastric cancer, which can be used as a new marker for the diagnosis of gastric cancer.

Key Words:  Serum; miR-101; miR-204; Gastric cancer; Diagnosis; Helicobacterpylori; Infection