作者：洪永刚1，郑浩2，黄智平3，刘启志1，张卫1，郝立强1

单位：1.海军军医大学长海医院 肛肠外科，上海 200433； 2.海军军医大学东方肝胆外科医院 肝外三科，上海 200433； 3.中国人民解放军南部战区总医院 肝胆外科，广东 广州 510010

Authors:  HONG Yonggang1, ZHENG Hao2, HUANG Zhiping3, LIU Qizhi1, ZHANG Wei1, HAO Liqiang1

Unit:  1.Department of Anorectal surgery, Changhai Hospital, Naval Military Medical University, Shanghai 200433, China； 2.Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Naval Military Medical University, Shanghai 200433, China； 3.Department of Hepatobiliary Surgery, General Hospital of Southern Theatre Command, Guangzhou 510010, Guangdong, China

摘要：

目的  探讨在结直肠癌中MicroRNA(miR)-875-5-875-5p的表达水平、细胞功能及调控机制。方法  通过q RT-PCR技术检测miR-875-5p在62例结直肠癌患者组织样本和永生化细胞器中的表达水平,功能学实验揭示miR-875-5p对结直肠癌细胞增殖和凋亡能力的影响。双荧光素酶和免疫印迹实验研究miR-875-5p与靶基因ELL2蛋白表达量之间的关系。结果  miR-875-5p在结直肠癌组织和结直肠癌细胞系中异常高表达。体外功能实验证实miR-875-5p可增强结直肠癌细胞增殖能力,同时降低结直肠癌细胞凋亡。双荧光素酶和免疫印迹实验证实miR-875-5p直接靶向负向ELL2-3’UTR区并抑制其蛋白的表达。结论  miR-875-5p在结直肠癌组织中高表达,miR-875-5p可促进结直肠癌细胞的增殖和抑制凋亡,其机制与下调ELL2表达有关。

关键词： 结直场癌; MicroRNA; 增殖; 凋亡; ELL2

Abstract：

Objective  To investigate the expression level, cell function and regulation mechanism of microRNA-875-5p in colorectal cancer (CRC). Methods  The expression of microRNA-875-5p in 62 paired CRC tissues and CRC cells was detected by qRT-PCR. Functional experiments revealed the effect of microRNA-875-5p on the proliferation and apoptosis of CRC cells. Double luciferase and Western blotting were used to study the relationship between ELL2 and microRNA-875-5p. Results  The expression of microRNA-875-5p was abnormally high in CRC tissues and CRC cell lines. Functional experiments in vitro confirmed that microRNA-875-5p could promote the proliferation and inhibit the apoptosis of CRC cells. Double luciferase and Western blotting experiments confirmed that microRNA-875-5p directly targeted the ELL2-3'UTR region and inhibited its expression. Conclusion  MicroRNA-875-5p is highly expressed in colorectal cancer tissues. MicroRNA-875-5p can promote the proliferation and inhibit apoptosis of colorectal cancer cells, and its mechanism is related to down-regulation of ELL2 expression.

Key Words:  Colorectal carcinoma; MicroRNA; Proliferation; Apoptosis; ELL2