作者：谢文轩1，孙开宇2，陈斌1，彭宝岗1，沈顺利1

单位：1.中山大学附属第一医院 肝外科，广东 广州 510080； 2.中山大学附属第一医院 胃肠外科中心，广东 广州 510080

Authors:  XIE Wenxuan1, SUN Kaiyu2, CHEN Bin1, PENG Baogang1, SHEN Shunli1

Unit:  1.Department of Hepatic Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong, China;  2. Department of Gastrointestinal Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong, China

摘要：

目的  检测长链非编码RNA-uc003 ykw.1 (lncRNA-uc003 ykw.1)在肝细胞癌组织中的表达并探究其对预后的影响。方法  实时荧光定量PCR(qRT-PCR)检测74对肝细胞癌组织及癌旁组织中lncRNA-uc003 ykw.1的表达,将病例分为高表达和低表达组,分析其与临床指标的关系,Kaplan-Meier曲线分析其预后,COX比例风险模型进行预后的单因素分析和多因素分析。结果  肝细胞癌中lncRNA-uc003 ykw.1表达量显著小于癌旁组织(P<0.05),lncRNA-uc003 ykw.1的表达与肿瘤最大直径、早期复发相关(P<0.05),单因素和多因素分析均表明lncRNA-uc003 ykw.1低表达是肝细胞癌患者预后不佳的独立预后因素。结论 lncRNA-uc003 ykw.1有望成为预测肝细胞癌预后的重要指标。

关键词： 长链非编码RNA-uc003 ykw.1; 肝细胞癌; 预后

Abstract：

Objective  To test the expression of long non-coding RNA uc003 ykw.1 (lncRNA-uc003 ykw.1) in hepatocellular carcinoma (HCC)and investigate its influence on prognosis. Methods Quantitive realtime PCR (qRT-PCR) was used to detect the expression of lncRNA-uc003 ykw.1 in 74 pairs of hepatocellular carcinoma tissues and adjacent tissues, which were divided into high expression group and low expression group. The relationship between lncRNA-uc003 ykw.1 and clinical characteristics was analyzed. Kaplan-Meier curve was performed to predict clinical prognosis. The univariete and multivariate analysis were carried out by COX hazard model. Results  The expression of lncRNA-uc003 ykw.1 in hepatocellular carcinoma tissues was significantly lower than that of the adjacent tissues (P＜0.05), and it was related with maximum diameter and early recurrence (P＜0.05), COX hazard model showed that the low expression of lncRNAuc003 ykw.1 was an independent poor prognostic factor for HCC patients. Conclusion  lncRNA-uc003 ykw.1 is expected to be an important marker to predict the prognosis of HCC.

Key Words:  Long non-coding RNA uc003 ykw.1; Hepatocellular carcinoma; Prognosis