作者：李志发，陈戎，吴小兵，黎淑玲

单位：广州医科大学附属第三医院 胃肠外科，广东 广州 510150

Authors:  LI Zhifa, CHEN Rong, WU Xiaobing, LI Shuling

Unit:  Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, Guangdong, China

摘要：

目的  分析miR-23a和miR-125b作为结直肠癌(Colorectal Cancer, CRC)诊断标记的潜能,并分析肿瘤分期对miR-23a和miR-125b表达水平的影响。方法  采用实时定量PCR(RT-q PCR)分析miR-23a在CRC患者肿瘤和瘤旁组织之间的表达谱,并分别分析miR-23a和miR-125b在Ⅱ、Ⅲ期CRC中的表达水平。结果  miR-23a和miR-125b在Ⅱ、Ⅲ期CRC患者及混合样本中的均为下调表达,且miR-125b在三组结直肠中下调表达均具有统计学意义(P<0.05)。结论  miR-23a和miR-125b具有作为结直肠癌诊断标记的潜能,但肿瘤分期对miR-23a和miR-125b的表达水平不一定具有明显影响。

关键词： 结直肠肿瘤; miR-23a; miR-125b; 生物标记; 诊断标记

Abstract：

Objective  Aim to confirm miR-23a and miR-125b as potential diagnostic markers of colorectal cancer (CRC), and evaluate the effect of tumor staging using the miR-23a and miR-125b expression level. Method  Real-time quantitative PCR (RT-PCR) analysis of the expression profile of miR-23a in the CRC tumors and adjacent tumor organizations, and were analyzed miR-23a and miR-125b expression levels at stage II, III CRC. Result  Both miR-23a and miR-125b were down-regulated in stage II, III CRC tumors and mixed samples, and miR-125b was down-regulated in all three groups (P<0.05). Conclusions  MiR-23a and miR-125b are potentially diagnostic markers for CRC, but the tumor staging does not necessarily have a significant effect on the expression levels of miR-23a and miR-125b.

Key Words:  Colorectal Cancer; miR-23a; miR-125b; Biomarkers; Diagnostic marker