作者：雷子颖1，王俞1，罗嘉莉2，阮强1，王佳泓1，唐鸿生1

单位：1.广州医科大学附属肿瘤医院 腹部综合外科，广东 广州 510095； 2.广州医科大学 肿瘤学系，广东 广州 510095

Authors:  LEI Ziying1, WANG Yu1, LUO Jiali2, RUAN Qiang1, WANG Jiahong1, TANG Hongsheng1

Unit: 1. Department of Abdominal Surgery, Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou 510095, Guangdong, China； 2. Department of oncology, Guangzhou medical university, Guangzhou 510095, Guangdong, China

摘要：

目的  研究胃癌患者外周血T淋巴细胞亚群以及自然杀伤细胞(natural killer cell,NK)数的变化及其临床意义。方法  使用全自动流式细胞仪检测68例胃癌患者外周血中T淋巴细胞亚群CD3+T细胞、CD4+T细胞、CD4+/CD8+和NK细胞水平,并将其与对照组32例健康人群进行比较分析。结果  胃癌患者与对照组健康人群相比,CD3+T细胞、CD4+T细胞、CD4+/CD8+比值以及NK细胞均明显下降(P<O.05),CD8+T细胞则明显升高(P<0.05)。随着胃癌患者TNM分期进展,肿瘤病灶大于5cm和淋巴结转移,CD3+T细胞、CD4+T细胞及NK细胞均进一步下降(P<0.05);CD8+T细胞则进一步升高;CD4+/CD8+比值亦下降。结论  胃癌患者细胞免疫功能低下,且与肿瘤分期相关,分期越晚,免疫功能越低。

关键词： 胃癌; T淋巴细胞亚群; NK细胞

Abstract：

Objective  To study the changes of T-lymphocyte subsets and NK cell in peripheral blood from patients with gastric cancer and their clinical significance. Methods  Flow Cytometry was performed to count and analyze T-lymphocytes and NK cell in peripheral blood of 68 patients with gastric cancer and 32 normal controls. Results  Compared with the normal controls, the percentage of CD3+T cell, CD4+ T cell, NK cell and the ratio of CD4+/CD8+ decreased obviously in the case group (P<0.05). With the progress of patients with different TNM stage, tumor lesions larger than 5 cm and lymph node metastasis, the percentage of CD3+T cell, CD4+T cell and NK cell in advanced gastric cancer group were significantly lower than those in early gastric cancer group (P<0.05); while the percentage of CD8+T cell was significantly higher than those in early gastric cancer group (P<0.05); the ratio of CD4+/CD8+ also decreased obviously in the advanced gastric cancer group. Conclusion  Cellular immune function in patients with gastric cancer is obviously abnormal. A remarkable significance is found in the different TNM stage, the cellular immune function is lower in patients with poor stage.

Key Words:  Gastric cancer; T-Lymphocyte subsets; NK cell